Constrained Multistate Sequence Design for Nucleic Acid Reaction Pathway Engineering
Journal: Wolfe BR, Porubsky NJ, Zadeh JN, Dirks RM, Pierce NA , (). Constrained Multistate Sequence Design for Nucleic Acid Reaction Pathway Engineering J. Am. Chem. Soc. . 139(8), 3134–3144
Abstract:We describe a framework for designing the sequences of multiple nucleic acid strands intended to hybridize in solution via a prescribed reaction pathway. Sequence design is formulated as a multistate optimization problem using a set of target test tubes to represent reactant, intermediate, and product states of the system, as well as to model crosstalk between components. Each target test tube contains a set of desired \on-target" complexes
