Polymerase chain reaction based scaffold preparation for the production of thin, branched DNA origami nanostructures of arbitrary sizes
Journal: Pound E, Ashton JR, Becerril HA, Woolley AT , (). Polymerase chain reaction based scaffold preparation for the production of thin, branched DNA origami nanostructures of arbitrary sizes Nano Lett. . 9(12), 4302–4305
Abstract:Designs for DNA origami have previously been limited by the size of the available single-stranded genomes for scaffolds. Here we present a straightforward method for the production of scaffold strands having various lengths, using polymerase chain reaction amplification followed by strand separation via streptavidin-coated magnetic beads. We have applied this approach in assembling several distinct DNA nanostructures that have thin ( approximately 10 nm) features and branching points, making them potentially useful templates for nanowires in complex electronic circuitry.
