Dabby MPP 2011
From MPPWiki
(Created page with "= The Kinetics of Toehold-Mediated Four-Way Branch Migration = * Nadine Dabby, Ho-Lin Chen, Erik Winfree, Caltech == Abstract ==") |
(→Abstract) |
||
| Line 4: | Line 4: | ||
== Abstract == | == Abstract == | ||
| + | |||
| + | Many developments in DNA nanotechnology rely on three-way branch migration | ||
| + | as a mechanism to implement switches, circuits, motors, assembly and | ||
| + | amplification. Four-way branch migration, the process by which two | ||
| + | double-stranded oligonucleotides that share the same stem sequence | ||
| + | simultaneously exchange strands has been used to perform directional | ||
| + | motion via insertion, but the applications of the four-way branch | ||
| + | migration mechanism have not been fully explored. While three-way branch | ||
| + | migration reaction rates can be controlled over six orders of magnitude, | ||
| + | four-way branch migration may give us a greater range and finer resolution | ||
| + | control over this rate. | ||
| + | We have found that by designing the toeholds involved in a four-way branch | ||
| + | migration reaction, we can control the effective reaction rate over seven | ||
| + | orders of magnitude. We characterize the kinetics of DNA toehold-mediated | ||
| + | four-way branch migration and model it as a two-step process. The ability | ||
| + | to control the kinetics of these reactions will greatly facilitate the | ||
| + | programming of dynamic behaviors mediated by four-way branch migration. | ||
Latest revision as of 07:33, 13 June 2011
The Kinetics of Toehold-Mediated Four-Way Branch Migration
- Nadine Dabby, Ho-Lin Chen, Erik Winfree, Caltech
Abstract
Many developments in DNA nanotechnology rely on three-way branch migration as a mechanism to implement switches, circuits, motors, assembly and amplification. Four-way branch migration, the process by which two double-stranded oligonucleotides that share the same stem sequence simultaneously exchange strands has been used to perform directional motion via insertion, but the applications of the four-way branch migration mechanism have not been fully explored. While three-way branch migration reaction rates can be controlled over six orders of magnitude, four-way branch migration may give us a greater range and finer resolution control over this rate. We have found that by designing the toeholds involved in a four-way branch migration reaction, we can control the effective reaction rate over seven orders of magnitude. We characterize the kinetics of DNA toehold-mediated four-way branch migration and model it as a two-step process. The ability to control the kinetics of these reactions will greatly facilitate the programming of dynamic behaviors mediated by four-way branch migration.
